Lensfree Holographic Microscopy

Here is a list of advanced technologies licensed or developed by Holomic. For research contracts or collaboration please contact Ketaki Sood, Director of Product Marketing, , 310.443.2070.

Stand-alone Microscope

A compact and high-throughput alternative to traditional microscope that can fit into your pocket

Standalone 3Lensfree Holographic Microscope
Weighing less than 50 grams, this microscope can provide sub-cellular resolution over a field of view of 24 mm2 that is an order of magnitude larger than the imaging area of a typical 10X objective lens.

Selected Publications:

  • O. Mudanyali, D. Tseng, C. Oh, S.O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, Lab on a Chip (2010), DOI: 10.1039/C000453G
  • D. Tseng, O. Mudanyali, C. Oztoprak, S.O. Isikman, I. Sencan, O. Yaglidere and A. Ozcan, Lab on a Chip (2010), Cover Article, DOI:10.1039/C003477K
  • O. Mudanyali, C. Oztoprak, D. Tseng, A. Erlinger, and A. Ozcan, Lab on a Chip (2010), DOI: 10.1039/C004829A
  • T. Su, A. Erlinger, D. Tseng, and A. Ozcan, Analytical Chemistry (2010), DOI: 10.1021/AC101845Q

Pixel-Super Resolution Microscopes

Portable microscopes with deeply submicron resolution over an imaging area that is orders of magnitude larger than conventional microscopes

4Pixel Super Resolution Microscope

Pixel Lensfree Tomographic Microscope

By the cost of additional image acquisition, lensfree pixel super-resolution microscopy can achieve a Numerical Aperture (NA) of ~0.8-0.9 across a field of view (FOV) of more than 20 mm2 or an NA of ~0.1 across a FOV of ~18 cm2, which corresponds to an image with more than 1.5 gigapixels.

Moreover, together with advanced algorithms, this pixel-super resolution approach has been also utilized in the first lensfree tomographic imager and lensfree color microscopes, e.g., to analyze micro-scale targets in three dimensions and image Pap smear (Papanicolaou test), respectively. These microscopes provide a unique toolset to image and investigate deeply submicron features of microscopic samples with an exceptional throughput.

Selected Publications:

  • Bishara, U. Sikora, O. Mudanyali, T. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, Lab on a Chip (2011), DOI:10.1039/C0LC00684J
  • S.O. Isikman, W. Bishara, S. Mavandadi, F.W. Yu, S. Feng, R. Lau and A. Ozcan, Proceedings of the National Academy of Sciences (PNAS) (2011), DOI:10.1073/PNAS.1015638108
  • A. Greenbaum, W. Luo, T-W. Su, Z. Göröcs, L. Xue, S.O. Isikman, A.F. Coskun, O. Mudanyali, and A. Ozcan, Nature Methods (2012), DOI:10.1038/NMETH.2114
  • T-W. Su, L. Xue and A. Ozcan, Proceedings of the National Academy of Sciences (PNAS) (2012), DOI: 10.1073/PNAS.1212506109
  • A. Greenbaum, N. Akbari, A. Feizi, W. Luo, and A. Ozcan, PLoS ONE (2013), DOI:10.1371/journal.pone.0076475

Wide-Field Imaging of Single Nano-Particles and Viruses

Following a novel nano-lens self-assembly procedure, holographic microscopy can image single nano particles and viruses over an ultra large field-of-view

Lensfree Wide-Field Imaging of single Viruses and Nanoparticles

Electron microscopy is one of the gold standards to observe nanoscale objects as the scattering from an individual nanoparticle (e.g., virus) is quite weak at optical wavelengths. Utilizing lensfree holographic microscopy and self-assembled liquid nano-lenses, our high-throughput and on-chip approach provides a unique platform to image sub-100 nm particles across a large field-of-view of >20 mm2. In addition to single nano-particles that are as small as 60nm, we also imaged single H1N1 viruses and adenoviruses.

Selected Publications:

  • O. Mudanyali, E. McLeod, W. Luo, A. Greenbaum, A.F. Coskun, Y. Hennequin, C. Allier, and A. Ozcan, Nature Photonics (2013), DOI:10.1038/NPHOTON.2012.337
  • O. Mudanyali, W. Bishara, and A. Ozcan, Optics Express (2011), DOI: 10.1364/OE.19.017378
  • E. McLeod, W. Luo, O. Mudanyali, A. Greenbaum, and A. Ozcan, Lab Chip (2013) , DOI: 10.1039/C3LC50222H